LOCUS Exported 3071 bp ds-DNA circular SYN 16-FEB-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAd1127-06 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 3071) AUTHORS Xavier Danthinne TITLE Direct Submission JOURNAL Exported Thursday, Feb 16, 2017 from SnapGene 3.3.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3071 /organism="recombinant plasmid" /mol_type="other DNA" repeat_region 4..106 /label=Ad5 left ITR /note="Ad5 left ITR" /note="ITR" promoter 67..75 /note="ATF binding site" misc_feature 243..251 /function="Ad5 genome packaging" /note="A1" /note="- A1 repeat - consensus sequence: 5'-TTTG-N8-CG-3' - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 263..272 /function="Ad5 genome packaging" /note="A2" /note="- A2 repeat - consensus sequence: 5'-TTTG-N8-CG-3' - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 305..314 /function="Ad5 DNA packaging" /note="A3" /note="- A3 repeat - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 316..324 /function="Ad5 DNA packaging" /note="A4" /note="- A4 repeat - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 340..349 /function="Ad5 DNA packaging" /note="A5" /note="- A5 repeat - consensus sequence: 5'-TTTG-N8-CG-3' - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 362..370 /function="Ad5 DNA packaging" /note="A6" /note="- A6 repeat - consensus sequence: 5'-TTTG-N8-CG-3' - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" misc_feature 370..378 /function="Ad5 DNA packaging" /note="A7" /note="- A7 repeat - Ostapchuk and Hearing (2005) J Cell Biochem. 96:25-35" CDS 612..1034 /codon_start=1 /gene="pIX" /product="Ad5 pIX" /label=Ad5 pIX /note="Ad5 pIX" /db_xref="GI:33465835" /protein_id="AAQ19288.1" /translation="MSTNSFDGSIVSSYLTTRMPPWAGVRQNVMGSSIDGRPVLPANST TLTYETVSGTPLETAASAAASAAAATARGIVTDFAFLSPLASSAASRSSARDDKLTALL AQLDSLTRELNVVSQQLLDLRQQVSALKASSPPNAV" rep_origin complement(1110..1698) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1873..2688) /codon_start=1 /product="Kanamycin-R" /label=Kanamycin-R /note="Kanamycin-R" /note="origin: Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_signal 2751..2998 /label=Cos site /note="Cos site" /note="phage lambda COS site" ORIGIN 1 taacatcatc aataatatac cttattttgg attgaagcca atatgataat gagggggtgg 61 agtttgtgac gtggcgcggg gcgtgggaac ggggcgggtg acgtagtagt gtggcggaag 121 tgtgatgttg caagtgtggc ggaacacatg taagcgacgg atgtggcaaa agtgacgttt 181 ttggtgtgcg ccggtgtaca caggaagtga caattttcgc gcggttttag gcggatgttg 241 tagtaaattt gggcgtaacc gagtaagatt tggccatttt cgcgggaaaa ctgaataaga 301 ggaagtgaaa tctgaataat tttgtgttac tcatagcgcg taatatttgt ctagggccgc 361 ggggactttg accgtttacg tggagactcg cccaggtgtt tttctcaggt gttttccgcg 421 ttccgggtca aagttggcgt tttagatctt ctagacccgg gagcggccgc tgtcgacctg 481 caggatccga attcgatatc actagtggta ccagtactga aatgtgtggg cgtggcttaa 541 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 601 ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa 661 cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc 721 gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt 781 tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga 841 ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg 901 atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg 961 tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc 1021 ccaatgcggt ttaacggtcc gggccagagt ggccgagcaa aaggccagca aaaggccagg 1081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 1141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 1201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 1261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 1321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 1381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 1441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 1501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 1561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 1621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 1681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 1741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcggccaa 1801 gaaggccaga gtggcagaga ctgcattcga aaacgtttga attgataatt attatcattt 1861 gcgggtcaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc 1921 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc 1981 gaggcagttc cataggattg caagatcctg gtatcggtct gcgattccga ctcgtccaac 2041 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc 2101 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg 2161 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt 2221 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca 2281 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc 2341 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag 2401 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc 2461 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc 2521 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc 2581 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga 2641 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt 2701 attactgttt atgtaagcag acagttttat tgttcatgcg aaaacgtttg aattgataat 2761 tattatcatt tgcgggtcct ttccggcgat ccgccttgtt acggggcggc gacctcgcgg 2821 gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct tcgtcataac 2881 ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacagctg aaagcgagct 2941 ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcgg 3001 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagcgata 3061 tttaaattaa t //