LOCUS pAd1130 10062 bp ds-DNA circular SYN 09-OCT-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10062) AUTHORS OD260 Inc TITLE Direct Submission JOURNAL Exported Wednesday, Oct 9, 2019 from SnapGene 5.0.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10062 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory join(4411,4424..4429) /regulatory_class="polyA_signal_sequence" /note="Ad5 E4 polyA signal (Le Moullec et al, 1983, J. Virol. 48:127-134) " CDS complement(join(4522..4800,5512..5685)) /codon_start=1 /product="Ad5 E4 ORF6/7" /function="- E4 Orf6 forms a complex with E1B-55K, that degrades p53" /label=Ad5 E4 ORF6/7 /db_xref="GI:56160560" /protein_id="AP_000227.1" /translation="MTTSGVPFGMTLRPTRSRLSRRTPYSRDRLPPFETETRATILEDH PLLPECNTLTMHNAWTSPSPPVKQPQVGQQPVAQQLDSDMNLSELPGEFINITDERLAR QETVWNITPKNMSVTHDMMLFKASRGERTVYSVCWEGGGRLNTRVL" CDS complement(4801..5685) /codon_start=1 /product="Ad5 E4 34K" /function="- apoptosis- DNA damage response - RNA metabolism and transport - transformation " /label=Ad5 E4 ORF6 /label=Ad5 E4 34K /db_xref="GI:56160561" /protein_id="AP_000228.1" /translation="MTTSGVPFGMTLRPTRSRLSRRTPYSRDRLPPFETETRATILEDH PLLPECNTLTMHNVSYVRGLPCSVGFTLIQEWVVPWDMVLTREELVILRKCMHVCLCCA NIDIMTSMMIHGYESWALHCHCSSPGSLQCIAGGQVLASWFRMVVDGAMFNQRFIWYRE VVNYNMPKEVMFMSSVFMRGRHLIYLRLWYDGHVGSVVPAMSFGYSALHCGILNNIVVL CCSYCADLSEIRVRCCARRTRRLMLRAVRIIAEETTAMLYSCRTERRRQQFIRALLQHH RPILMHDYDSTPM" CDS complement(5606..5950) /codon_start=1 /product="Ad5 E4 ORF4" /function="- RNA splicing" /label=Ad5 E4 ORF4 /db_xref="GI:56160562" /protein_id="AP_000229.1" /translation="MVLPALPAPPVCDSQNECVGWLGVAYSAVVDVIRAAAHEGVYIEP EARGRLDALREWIYYNYYTERSKRRDRRRRSVCHARTWFCFRKYDYVRRSIWHDTTTNT ISVVSAHSVQ" CDS complement(5961..6311) /codon_start=1 /product="Ad5 E4 ORF3" /function="- DNA damage response- transformation - releases p53 from E1B 55K " /label=Ad5 E4 ORF3 /db_xref="GI:56160563" /protein_id="AP_000230.1" /translation="MIRCLRLKVEGALEQIFTMAGLNIRDLLRDILRRWRDENYLGMVE GAGMFIEEIHPEGFSLYVHLDVRAVCLLEAIVQHLTNAIICSLAVEFDHATGGERVHLI DLHFEVLDNLLE" CDS complement(6308..6700) /codon_start=1 /product="Ad5 E4 ORF 2(B) [ident:99.49, cov:100.00]" /label=Ad5 E4 ORF 2(B) /label=Ad5 E4 ORF 2 /protein_id="AP_000231.1" /translation="MFERKMVSFSVVVPELTCLYLHEHDYDVLSFLREALPDFLSSTLH FISPPMQQAYIGATLVSIAPSMRVIISVGSFVMVPGGEVAALVRADLHDYVQLALRRDL RDRGIFVNVPLLNLIQVCEEPEFLQS" CDS complement(6748..7134) /codon_start=1 /product="Ad5 E4 ORF1" /function="- E4 Orf1 is a transforming protein in a variety of human Ad serotypes but that this activity is manifest only when expression reaches a sufficient level (" /label=Ad5 E4 ORF1 /db_xref="GI:56160565" /protein_id="AP_000232.1" /translation="MAAAVEALYVVLEREGAILPRQEGFSGVYVFFSPINFVIPPMGAV MLSLRLRVCIPPGYFGRFLALTDVNQPDVFTESYIMTPDMTEELSVVLFNHGDQFFYGH AGMAVVRLMLIRVVFPVVRQASNV" promoter 7216 /label=E4 Transcription Start Site /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290 " regulatory 7242..7247 /regulatory_class="TATA_box" /note="Jones and Lee (1991), Mol Cell Biol 11:4297-4305 PMCID:PMC361290 " repeat_region complement(7444..7546) /label=Ad5 right ITR promoter 7592..7696 /gene="bla" /label=AmpR promoter CDS 7697..8557 /codon_start=1 /product="beta-lactamase" /label=Ampicillin-R /label=M_Ampicillin-r_* /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 8728..9316 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature join(9377..10062,1..4324) /product="Scrambled Lambda DNA" /label=Scrambled Lambda DNA ORIGIN 1 tggccgttac gccaccagtc atcctcacga tgaaagtccg gcatctcaat ccccgccaga 61 tgataagcat cccggaacag tgtgtaacag tccgtcaccg aaaactcctc cagtctgctg 121 gcgttatcct ccgtcagctc gactttttcc agcagctcct tgccgagatg ggattcggtt 181 atcttgcctt tgaaaaaatc caggtaacct tccgcatcat cgctcgcccg accgacggcc 241 tccacgaatg ccgatttgcc aacggtgttc acactgcgga tataaaagta ataatcatgg 301 cccggtttga tattgatact ggcggctatc cagtacagcg ccgtaccaag ataacgcgtg 361 ctggtttcaa cctgtctgat atccgcaatc tgcttttccg agaaccagaa ctcaaactgt 421 accgtcgggt cataaacggc aagatgcggc gtggcggtta tctgaaaata gcccggcgtc 481 agctcaatcc tcgacggtgc tgccggtgcg gcaatccgga acgataccga cgccggatcg 541 ccctgctgcc cccacgcatt taccgcccgg actgtcagcc tgtagttccc cagcgccagt 601 tgcgtgaagc ggtatgtggt ttccgtcgtc cgggccgtgc tgaccagccg ctcactgccg 661 tcgtccgctg ttacggtcag acggagcagg aaactcacgc ccttcaccac cttcggtgtg 721 tcccatcgcg ccagcacctg atattccccg ctgtctgcag tgacttctgc ggtcaggtgc 781 tgcaccgctg gcggcgtgac accattcacc gtgccactct gttcgccgtc aaagtgcgcc 841 ccgttatcca cgatggcctc tttttccggc acatgctgca cggcggtgat ggcatacgtg 901 ccgtcgtcgt tctcacggat actcacgcag cggaacagtc gctggcgcag cgtcggcagc 961 ttcagctccc atacgctgta ttcagcaaca ccgtcaggaa cacggctcac ttttaccttc 1021 acgccgtcgg tgacggactg aacctccacg ctgaccggat tgccacttcc gtcaaccagg 1081 cttatcagcg cggtaccgga ggatggcagc gtgatttcac ggtcgagcgt cagcgtccgg 1141 gtctggctgt tcaccgccag cacacgacca ccggtgctga taccggcata gtcatcatcg 1201 cagatttcaa taacatcgcc cggtacatgg cgaagccctt ctgcgccgac gctgaaatcc 1261 acggtctgcg tttccagcag ttctgtttta atcagccaca gcccggcgcg gtgtgcctgc 1321 ccccggctgg tacagccaaa ggcatccatc ttcgtaacat tacgaccgta acgggcaatg 1381 gcctgcgtat cttcaacaag ctctgtcgcc gtctcccagc cgttgttcgg gtcaatccag 1441 ttcacctcaa cggcattatg gcggtccttc agggcgctga agctgtagcg gaacggcgcg 1501 gaccgctata accgcactcg tcaccgcgat aggtccaggt gcaggtgttg gccagcatga 1561 tacgtcccgg aaaaacagcg ccatccgttt ccgtcggcgt ggacagtaca aaggaggcac 1621 tcaccgcgct cagttcgctg cactgctcaa tgcgccagcg gctgatcacc tcctgctccg 1681 gatcggcgta actgtttccg ttgacgaagt tcaccgcatc cagaaaacgg gcgtaaacct 1741 tacgccggac caccgttccg ccgaccagac tctgcatatc ttccgccatc ccggtgacca 1801 taccgtacag gttagaaacc gtcagcgtgg ggcgcgtact ggtgcctttg ccattcagtt 1861 caaaaccgct cccctgaatg ggatacggct gatactgtcg cccctgccag gtgaccggct 1921 cacctttttc gttctgctca ttacagaaaa aataacgttc tccaccgacc tctggtcagg 1981 tcgatttccc agagcaccac gctggccgac tgctccgcac gggtgcattc attcagtgtt 2041 tcctgccgga tatcctgcat cagttcacca cctgttcaaa ctctgcgctg aactcaacac 2101 gcagcatact gacccgcgac gaccattttg cgcaggtcac ctttatctgc cgccactcat 2161 aaggcggcgt ccacagaaag gatttccagc ccccgtgctc ttccagaaac gactccagta 2221 ccgtggcctc ctcacggggg acagaaagcg tcacgctgta cgttttcagg ttggcattca 2281 gcccggcagg cgctcgctga gaatagccat caccaaagcg cacctttctt acagaaggga 2341 ccgaagccac atccataccg ggtttcactt tccagcggaa ggtcttcatc gtccacctcc 2401 ggagaacagg ccaccatcac gcatctgtgt ctgaatttca tcacgggcac ccttgcgggc 2461 catgtcatac accgccttca gagcagccgg acctatctgc ccgttcgtgc cgtcgttgtt 2521 aatcaccaca tggttattct gctcaaacgt cccggacgcc tgcgaccggc tgtctgccat 2581 gctgcccggt gtaccgacat aaccgccggt ggcatagccg cgcatcagcc ggtaaagatt 2641 ccccacgcca atccggctgg ttgcctcctt cgtgaagaca aactcaccac ggtgaacaat 2701 ccccgctggc tcatatttgc cgccggttcc cgtaaatcct ccggttgcaa aatggaattt 2761 cgccgcagcg gcctgaatgg ctgtaccgcc tgacgcggat gcgccgccac caacagcccc 2821 gccaatggcg ctgccgatac tcccgacaat ccccaccatt gcctgcttaa gcagaatttc 2881 tgtcatcatg gacagcacgg aacgggtgaa gctgcgccag ttctgctcac tgccggtcag 2941 catcgccgcc atattctgtg caataccatc aaaggtctgc gtggctgcac tttttacctg 3001 cgacatactg tccgtggcgc tctcttccca ctcactccag ccggacttca ggcctgccat 3061 ccagttcccg cgaagctggt cttcagccgc ccaggtcttt ttctgctctg acatgacgtt 3121 attcagcgcc agcggattat cgccatactg ttccttcagg cgctgttccg tggcttcccg 3181 ttctgcctgc cggtcagtca gcccccggct tttcgcatca atggcggccc gttttgcccg 3241 ttgctgctgt gcgaatttat ccgcctgctg cgccagcgcg ttcaggcgct cctgatacgt 3301 aaccttgtcg ccaagtgcag ccagctggcg tttgtactcc agcgtctcat ctttatgcgc 3361 cagcagggat ttctcctgtg cagacagctg gcgacgttgc gccgcctcct ccagtaccgc 3421 gaactgactc tccgccttcc acaaatcccg gcgctgctgg ctgattttct catttgctcc 3481 ggcatgcttc tccagcgtcc ggagttctgc ctgaagcgtc agcagggcag catgagcact 3541 gtcttcctga cgatcgcccg cagacacctt cacgctggac tgtttcggct ttttcagcgt 3601 cgcttcataa tcctttttcg ccgccgccat cagcgtgttg taatccgcct gcaggatttt 3661 cccgtctttc agtgccttgt tcagttcttc ctgacgggcg gtatatttct ccagcggcgt 3721 ctgcagccgt tcgtaagcct tctgcgcctc ttcggtatat ttcagccgtg acgcttcggt 3781 atcgctctgc tgctgcgcat ttttgtcctg ttgagtctgc tgctcagcct tctttcgggc 3841 ggcttcaagc gcaagacggg ccttttcacg atcatcccag taacgcgccc gcgcttcatc 3901 gttaacaaaa taatcatcct tgcgcagatt ccagatgtcg tctgctttct tatacgcagc 3961 ctctgcctta atcagcatct cctgcgcggt atcaggacga ccaatatcca gcaccgcatc 4021 ccacatggat ttgaatgccc gcgcagtcct gtctgcccag gtctccagcg tgcccatgtt 4081 ctctttcagg cggcgggtct ggtcatcaaa ccctttcgtt gcggcctcgt tcgccgcctg 4141 caatgccccg gcttcatcgc cggaacgctg caactgagca acatacgcaa tctgctccgc 4201 cgacacgtta tggaactggc gagccatcgc cgtcagcccc gacgtcgggt ctgtggtcag 4261 cttcccgaag gcttcagcga cctttgtcca cctccacgcc ggatgcagag gagaaacgcg 4321 ccacgatctt ctagaggcct agaaggccac atgacaagga ctatgtacta cgagtccaat 4381 acactcatgg aggacggcca ccatggcctt atgtttcaac gtgtttattt ttcaattgca 4441 gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata gcttatacag 4501 atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac ctccctccca 4561 acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt 4621 aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc 4681 agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg 4741 agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc 4801 ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc 4861 gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc 4921 ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg cacagcagcg 4981 caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa 5041 aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg 5101 gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat 5161 aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg 5221 attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc 5281 tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc 5341 atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca 5401 cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa caacccattc 5461 ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat 5521 tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtttc 5581 tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca accgagatcg 5641 tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctgaagcaaa 5701 accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta gatcgctctg 5761 tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg gcttcgggtt 5821 ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca gaataagcca 5881 cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg ggaagagctg 5941 gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa atgaagatct 6001 attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa agaacagata 6061 atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct cacgtccaag 6121 tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc agcaccttca 6181 accatgccca aataattctc atctcgccac cttctcaata tatctctaag caaatcccga 6241 atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt cagcctcaag 6301 cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga ttcaaaagcg 6361 gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc tgaacataat 6421 cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg acaaaagaac 6481 ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc ccgatgtaag 6541 cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca ggcaaagcct 6601 cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag gtaagctccg 6661 gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt ttctgcataa 6721 acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta caacaggaaa 6781 aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt aaaaaaactg 6841 gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag tcataatgta 6901 agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc gaccgaaata 6961 gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca taggaggtat 7021 aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct gcctaggcaa 7081 aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag ccataacagt 7141 cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct 7201 caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa 7261 tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca 7321 gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta 7381 cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa 7441 aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca 7501 ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa ttaatttaaa 7561 tatcgcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 7621 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 7681 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 7741 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 7801 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 7861 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 7921 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 7981 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 8041 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 8101 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 8161 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 8221 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 8281 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 8341 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 8401 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 8461 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 8521 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 8581 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 8641 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 8701 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 8761 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 8821 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 8881 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 8941 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 9001 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 9061 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 9121 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 9181 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 9241 tcctgttggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 9301 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 9361 gccttttgct cacatgtacg gggagttctg gctttcggtg ccagcatctg cgccacacca 9421 ccgagcacca tactggcacc gagagaaaac aggatgccgg tcataccacc ggccccaatg 9481 gctgcccccc atgctgcaag ggtggctccg gcggtaaaga atgatccggc aatggcggca 9541 gcccccagga caatctggaa tacgccacct gacttggccc cggcgactct gggaacaata 9601 tgaattacag cgccatcagg cagagtctca tgtaactgcg ccgttaaccc ggacgtgctg 9661 acgtcccgcc cggcaatccg tacctgatac cagccgtcgc tcagtttctg acgaaacgcc 9721 gggagctgtg tggccagtgc ccggatggct tcagcccccg ttttcacacg aaggtcgatg 9781 cggcgaccaa atcgttgtaa atccccgtaa aggcagatgc gcgcgcgcca tgcccggtga 9841 cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtgt acctctctcg tttgctcagt 9901 tgttcaggaa tatggtgcag cagctcgccg tcgccgcagt aaattgcggc gtgattcggc 9961 actgatgaac caaaacagca cagcagcaca tcgcccggct gtgccgctga caacggcacc 10021 tgatacagcc ccgtcgcctc cagattatcc agatagagat tc //