LOCUS Exported 4189 bp ds-DNA circular SYN 05-DEC-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4189) AUTHORS Xavier Danthinne TITLE Direct Submission JOURNAL Exported Dec 5, 2017 from SnapGene 4.1.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4189 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 9..288 /codon_start=1 /product="Ad5 E3 12.5K" /label=Ad5 E3 12.5K (truncated) /label=Ad5 E3 12.5K /db_xref="GI:56160551" /protein_id="AP_000218.1" /translation="MLSGEAEQLRLKHLVHCRRHKCFARDSGEFCYFELPEDHIEGPAH GVRLTAQGELARSLIREFTQRPLLVERDRGPCVLTVICNCPNPGLHQD" misc_feature 290..358 /label=MCS-1 /note="Multiple cloning site" CDS complement(408..571) /codon_start=1 /product="Ad5 E3 U Exon" /label=Ad5 E3 U Exon /db_xref="GI:56160558" /protein_id="AP_000225.1" /translation="MKIVGADGQEQEETDIPFRLWRKFAARRKLQYQSWEEGKEVLLNK LDRNLLTDF" CDS 582..2345 /codon_start=1 /product="Ad5 protein IV, which trimerizes to form the fiber. The fiber and protein III form the penton base located at the vertices of the icosahedron." /label=Ad5/3 Fiber /label=Ad5 Fiber /db_xref="GI:56160559" /protein_id="AP_000226.1" /translation="MKRARPSEDTFNPVYPYDTETGPPTVPFLTPPFVSPNGFQESPPG VLSLRLSEPLVTSNGMLALKMGNGLSLDEAGNLTSQNVTTVSPPLKKTKSNINLEISAP LTVTSEALTVAAAAPLMVAGNTLTMQSQAPLTVHDSKLSIATQGPLTVSEGKLALQTSG PLTTTDSSTLTITASPPLTTATGSLGIDLKEPIYTQNGKLGLKYGAPLHVTDDLNTLTV ATGPGVTINNTSLQTKVTGALGFDSQGNMQLNVAGGLRIDSQNRRLILDVSYPFDAQNQ LNLRLGQGPLFINSAHNLDINYNKGLYLFTASNNSKKLEVNLSTAKGLMFDATAIAINA GDGLEFGSPNAPNTNPLKTKIGHGLEFDSNKAMVPKLGTGLSFDSTGAITVGNKNNDKL TLWTGPKPEANCIIEYGKQNPDSKLTLILVKNGGIVNGYVTLMGASDYVNTLFKNKNVS INVELYFDATGHILPDSSSLKTDLELKYKQTADFSARGFMPSTTAYPFVLPNAGTHNEN YIFGQCYYKASDGALFPLEVTVMLNKRLPDSRTSYVMTFLWSLNAGLAPETTQATLITS PFTFSYIREDD" promoter join(602,688..692) /label=U exon Promoter /note="- This proximal inverted CCAAT box located 86 bp upstream of the U exon transcription start site is critical for the promoter to function. The E2F sites at position -123 and -387 relative to the transcription start site are not critical (Ying et al, 2010, J Virol 84:11470-8)." regulatory join(2344..2349,2359) /regulatory_class="polyA_signal_sequence" /note="Ad5 L5 polyA signal (Le Moullec et al, 1983, J. Virol. 48:127-134)" rep_origin complement(2430..3018) /direction=LEFT /label=pUC19 Ori CDS complement(3189..4049) /codon_start=1 /product="beta-lactamase" /label=Ampicillin-R /label=M_Ampicillin-r_* /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ORIGIN 1 atgaggccat gttaagtgga gaggcagagc aactgcgcct gaaacacctg gtccactgtc 61 gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa ttgcccgagg 121 atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag cttgcccgta 181 gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg ggaccctgtg 241 ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatcca gatcttctag 301 acccgggagc ggccgctgtc gacctgcagg atccgaattc gatatcacta gtggtaccga 361 tcttattccc tttaactaat aaaaaaaaat aataaagcat cacttactta aaatcagtta 421 gcaaatttct gtccagttta ttcagcagca cctccttgcc ctcctcccag ctctggtatt 481 gcagcttcct cctggctgca aactttctcc acaatctaaa tggaatgtca gtttcctcct 541 gttcctgtcc atccgcaccc actatcttca tgttgttgca gatgaagcgc gcaagaccgt 601 ctgaagatac cttcaacccc gtgtatccat atgacacgga aaccggtcct ccaactgtgc 661 cttttcttac tcctcccttt gtatccccca atgggtttca agagagtccc cctggggtac 721 tctctttgcg cctatccgaa cctctagtta cctccaatgg catgcttgcg ctcaaaatgg 781 gcaacggcct ctctctggac gaggccggca accttacctc ccaaaatgta accactgtga 841 gcccacctct caaaaaaacc aagtcaaaca taaacctgga aatatctgca cccctcacag 901 ttacctcaga agccctaact gtggctgccg ccgcacctct aatggtcgcg ggcaacacac 961 tcaccatgca atcacaggcc ccgctaaccg tgcacgactc caaacttagc attgccaccc 1021 aaggacccct cacagtgtca gaaggaaagc tagccctgca aacatcaggc cccctcacca 1081 ccaccgatag cagtaccctt actatcactg cctcaccccc tctaactact gccactggta 1141 gcttgggcat tgacttgaaa gagcccattt atacacaaaa tggaaaacta ggactaaagt 1201 acggggctcc tttgcatgta acagacgacc taaacacttt gaccgtagca actggtccag 1261 gtgtgactat taataatact tccttgcaaa ctaaagttac tggagccttg ggttttgatt 1321 cacaaggcaa tatgcaactt aatgtagcag gaggactaag gattgattct caaaacagac 1381 gccttatact tgatgttagt tatccgtttg atgctcaaaa ccaactaaat ctaagactag 1441 gacagggccc tctttttata aactcagccc acaacttgga tattaactac aacaaaggcc 1501 tttacttgtt tacagcttca aacaattcca aaaagcttga ggttaaccta agcactgcca 1561 aggggttgat gtttgacgct acagccatag ccattaatgc aggagatggg cttgaatttg 1621 gttcacctaa tgcaccaaac acaaatcccc tcaaaacaaa aattggccat ggcctagaat 1681 ttgattcaaa caaggctatg gttcctaaac taggaactgg ccttagtttt gacagcacag 1741 gtgccattac agtaggaaac aaaaataatg ataagctaac cctatggaca ggtccaaaac 1801 cagaagccaa ctgcataatt gaatacggga aacaaaaccc agatagcaaa ctaactttaa 1861 tccttgtaaa aaatggagga attgttaatg gatatgtaac gctaatggga gcctcagact 1921 acgttaacac cttatttaaa aacaaaaatg tctccattaa tgtagaacta tactttgatg 1981 ccactggtca tatattacca gactcatctt ctcttaaaac agatctagaa ctaaaataca 2041 agcaaaccgc tgactttagt gcaagaggtt ttatgccaag tactacagcg tatccatttg 2101 tccttcctaa tgcgggaaca cataatgaaa attatatttt tggtcaatgc tactacaaag 2161 caagcgatgg tgcccttttt ccgttggaag ttactgttat gcttaataaa cgcctgccag 2221 atagtcgcac atcctatgtt atgacttttt tatggtcctt gaatgctggt ctagctccag 2281 aaactactca ggcaaccctc ataacctccc catttacctt ttcctatatt agagaagatg 2341 actaataaag aatcgtttgt gggccaccat ggccgagcaa aaggccagca aaaggccagg 2401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cgcgctcgtc gtttggtatg 3541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4141 ataggggttc cgcgcacatt ccccgaaaag tgccacctga cgtcggcca //