We offer two methods to determine the infectious titer of an adenovirus stock.
- TCID50 method: Cell monolayers are seeded in multi-well plates and are infected with a serial dilution of the virus suspension to be titrated. Positive wells are counted generally from 12 to 18 days after the infection. This is the most accurate and precise method.
- Plaque assay: Cell monolayers are seeded in tissue culture dishes. They are infected with a serial dilution of the virus suspension to be titrated, and covered with agar. Plaques are counted generally from 12 to 18 days after the infection.
- Determination of the concentration of infectious units (IU) in a crude or purified virus stock.
- A purified virus stock or a crude virus lysate
The service includes
- Infection of cell monolayers with a serial dilution of your virus
- Determination of titer (IU/mL)
- A complete report describing the work performed, including the raw data and the determination of the titer
- 12-18 days, depending on how fast the virus replicates.
Important Note on Handling Adenovirus
The use of adenovirus as a genetic vector requires adequate containment equipment and practices. Biosafety Level 2 (BL2) is appropriate for most customers; however, for some, use of BL3 or higher level containment facility may be required. PLEASE NOTE, O.D.260 Inc. will not carry out projects that require BL3 (or above) containment.