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  • Reagents for Cosmid Construction

Cosmid Construction Kit-1

Reagents for Adenovirus Cosmid Construction with AdenoQuick1.0

  • Sufficient for constructing at least 10 vectors (that is only $8.50 per construct)
  • Reagents are automatically added to your order when you buy the AdenoQuick kits
  • Contents:
    • maltose/MgSO4(# R-1023)
    • SM medium (# R-1004)
    • λ packaging extract (#R-1005)
    • E. coli OD101 glycerol stock (#R-1006)
    • Amp/Kan antibiotic mix (#R-1007)
  • Storage: - 70 ºC
$85.00
Cat# :
R-1001
Size: 
10 reactions

The Cosmid Construction Kit-1 contains most of the reagents necessary to construct AdenoQuick cosmids:

  • OD101 is the E. coli strain used to construct and amplify adenovirus cosmid DNAs.
  • Maltose and MgSO4 are added to the E. coli culture to induce expression of the receptor for phage λ
  • The λ packaging extract is used to package the DNA ligation products into the phage λ heads.
  • The SM medium is used to stop the packaging reaction at the end of the 30-minutes incubation period.
  • The Amp/Kan antibiotic mix will be used to select for colonies containing the adenovirus cosmid.

The reagents that the user needs to provide are:

  • T4 DNA ligase
  • Chloroform
  • LB Lennox (liquid and petri dishes)
ProductCat#SizePrice

Cosmid Construction Kit-2

Reagents for Adenovirus Cosmid Construction with AdenoQuick2.0
Cat# :
R-1002
10 reactions
$105.00
More Info »
Product Information Sheet
Product_Information_Cosmid_Construction_Kit-1.pdf (260.59 KB)