pZAP1.1
Shuttle plasmid for the construction of adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation)
- Insertion of heterologous sequences in place of the E1 region of the Ad5 genome ("first-generation" adenovirus vectors)
- E3 and/or E4 regions WT or deleted
- Ad5, Ad5/35, or Ad5/3 knob
$220.00
Cat# :
ZP-01 Size:
20 µg pZAP1.1 is a 3.5 kb shuttle plasmid designed for inserting expression cassettes in place of the E1 region of the Ad5 genome, in combination with RightZAP1.1 (WT E3), RightZAP1.2 (ΔE3), RightZAP1.3 (ΔE3 + Ad5/35 knob) and other vectors from the AdenoZAP1.0 cloning system. It contains a multiple cloning site downstream from the left ITR and packaging signal of the Ad5 genome. Expression cassettes inserted into this site should contain a promoter, cDNA, and polyA signal. The left arm DNA (which corresponds to the left ITR, packaging signal and expression cassette) can be excised from the vector with either PacI or SwaI on one side, and either PflMI, DraIII, SfiI, BstAPI or AlwNI on the other side.
Product | Cat# | Size | Price | |
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pZAP1.2Shuttle plasmid for the construction of adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation or recombination) |
Cat# :
ZP-03 |
10 µg |
$220.00 |
|
RightZAP1.1 DNALinear 32.5 kb Ad5 DNA for the construction of E1-deleted adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation or recombination) |
Cat# :
ZR-01 |
10 reactions |
$450.00 |
|
RightZAP1.2 DNALinear 30 kb Ad5 DNA for the construction of E1/E3-deleted adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation or recombination) |
Cat# :
ZR-02 |
10 reactions |
$450.00 |
|
RightZAP1.3 DNALinear 29 kb Ad5 DNA for the construction of E1/E3-deleted Ad5/35 adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation or recombination) |
Cat# :
ZR-05 |
10 reactions |
$550.00 |
|
RightZAP1.4 DNALinear 27.8 kb Ad5 DNA for the construction of E1/E3/E4-deleted Ad5/35 adenovirus vectors with the AdenoZAP1.0 cloning system (in vitro ligation or recombination) |
Cat# :
ZR-06 |
10 reactions |
$550.00 |
Vector Map | Polylinker | Sequence | Product Information Sheet |
---|---|---|---|
Vector Map | pZAP1-1_MCS.png (383.95 KB) | pZap11.txt (4.57 KB) | Product_Information_pZAP11.pdf (48.42 KB) |