Replication-deficient Ad5 with CMV-βGal cassette in place of the E1 region
- Strong expression of nuclear-targeted βGalactosidase
- E1/E3 deleted Ad5 backbone
- purified by double CsCl banding
- A total of 5 x 1011 VP will be provided in 100 µL aliquots. The number of vials you will receive will depend on the virus concentration. Contact us for more information.
Ad5.CMVβGal is an Ad5-based adenovirus containing a 4.3 kb-long LacZ expression cassette in place of the E1 region, oriented from right to left relative to the adenovirus genome. The E. coli β-galactosidase coding sequence is fused to a SV40 nuclear localization signal (GPKKKRKVGS) and is under the control of a human cytomegalovirus (CMV) promoter and a mouse protamine 1 polyadenylation signal. Both E1 (3.1 kb) and E3 (2.7 kb) regions are deleted. The two SfiI sites naturally present in WT Ad5 DNA were mutated by substituting A for G and C at positions 16291 and 16294 in the Ad5 genome, and C and G for respectively G and C at positions 23001 and 23004 in the Ad5 genome, introducing silent mutations in the adenovirus pVII and DNA-binding protein coding sequences.
|Vector Map||Sequence||Product Information Sheet|
|Vector Map||AdCMVbGal_#Ad103S.txt (33.69 KB)||Product_Information_Ad5CMVbGal_#Ad103S.pdf (139.97 KB)|