pAd1130-04

Shuttle plasmid for constructing adenovirus vectors with a 1.2 kb deletion in the E4 region

  • 1.2 kb SmaI-BglII E4 deletion (ORF1-4: same as in plasmids pAd361, pAd362, and pAd363)
  • E1/E3/E4-deleted adenovirus vectors with max 8.9 kb cargo capacity
  • AdenoQuick2.0 cloning system
  • Sequence-verified

 

$250.00
Cat# :
QP-19
Size: 
20 µg

pAd1130-04 is a plasmid designed for constructing recombinant adenovirus vectors, in combination with the AdenoQuick2.0 plasmids pAd1127, pAd1128, pAd1129, and their derivatives. It is a derivative of pAd1130 with a 1.2 kb SmaI-BglII E4 deletion that encompasses ORF 1-4, and corresponds to bp 34119-35355 in the WT Ad5 genome.
pAd1130-04 includes the right ITR and a 1.9 kb-long fragment of the E4 region. The right ITR is flanked with PacI and SwaI sites.  The E4 region is terminated with two SfiI sites, which generate non-symmetrical sticky ends suitable for directional cloning. The plasmid contains a 5 kb stuffer made from scrambled phage λ DNA.  This stuffer increases the size of the ligation product of pAd1127, pAd1128, pAd1129, and pAd1130 so that it can be packaged efficiently into phage λ.

ProductCat#SizePrice

pAd1130 Updated

Shuttle vector for the construction of adenovirus vectors using the AdenoQuick2.0 system (WT E4 region)
Cat# :
QP-13
20 µg
$250.00
More Info »

pAd1130-01

Shuttle plasmid for constructing adenovirus vectors with the E4 region under the control of a heterologous promoter
Cat# :
QP-14
20 µg
$250.00
More Info »

pAd1130-02

Shuttle plasmid for constructing adenovirus vectors with the E4 region under the control of a heterologous promoter with its own TATA box
Cat# :
QP-15
20 µg
$250.00
More Info »

pAd1130-03 Updated

Shuttle plasmid for constructing adenovirus vectors with a 2.8 kb deletion in the E4 region
Cat# :
QP-16
20 µg
$250.00
More Info »
Vector MapSequenceProduct Information Sheet
Vector Map pAd1130-04.txt (8.83 KB) Product Information pAd1130-04.pdf (214.91 KB)