Cosmid Construction Kit-2
Reagents for Adenovirus Cosmid Construction with AdenoQuick2.0
- Sufficient for constructing at least 10 vectors (that is only $10.50 per construct)
- Reagents are automatically added to your order when you buy the AdenoQuick2.0 kits
- Contents:
- maltose/MgSO4(# R-1023)
- SM medium (# R-1004)
- λ packaging extract (#R-1005)
- E. coli OD101 glycerol stock (#R-1006)
- Amp/Kan antibiotic mix (#R-1007)
- SfiI enzyme (#R-1008)
- SfiI 10x buffer (#R-1009)
- Storage: -20 ºC for SfiI enzyme and -70 ºC for lambda packaging extract
$105.00
Cat# :
R-1002 Size:
10 reactions The Cosmid Construction Kit -2 contains most of the reagents necessary to construct AdenoQuick2.0 cosmids:
- The SfiI restriction enzyme is used to digest the 4 shuttle plasmids before ligating them to each other.
- OD101 is the E. coli strain used to construct and amplify adenovirus cosmid DNAs.
- Maltose and MgSO4 are added to the E. coli culture to induce expression of the receptor for phage λ
- The λ packaging extract is used to package the DNA ligation products into the phage λ heads.
- The SM medium is used to stop the packaging reaction at the end of the 30-minutes incubation period.
- The Amp/Kan antibiotic mix will be used to select for colonies containing the adenovirus cosmid.
The reagents that the user needs to provide are:
- T4 DNA ligase
- Chloroform
- LB Lennox (liquid and petri dishes)
The SfiI restriction enzyme must be stored at - 20 ºC. The lambda packaging extract must be stored at - 70 ºC.
| Product | Cat# | Size | Price |
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| Vector Map | Product Information Sheet |
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| Vector Map | Product_Information_Cosmid_Construction_Kit-2.pdf (262.85 KB) |