Plasmid-isolated genome of a Ad5 replication-deficient vector containing a CMV-βGal expression cassette in place of the E1 region
- Used as positive control for transfections
- The β-Galactosidase coding sequence is fused to a SV40 large tumor nuclear localization signal.
- β-Galactosidase expression is under the control of a CMV promoter and a mouse protamine 1 polyA signal.
- Allows for at least 5 transfections.
Ad5.CMVβGal DNA is the plasmid-isolated genome of an Ad5-based adenovirus containing a 4.3 kb-long LacZ expression cassette in place of the E1 region, oriented from right to left relative to the adenovirus genome. The E. coli β-galactosidase coding sequence is fused to a SV40 nuclear localization signal (GPKKKRKVGS) and is under the control of a human cytomegalovirus (CMV) promoter and a mouse protamine 1 polyadenylation signal. Both E1 (3.1 kb) and E3 (2.7 kb) regions are deleted. The two SfiI sites naturally present in WT Ad5 DNA were mutated by substituting A for G and C at positions 16291 and 16294 in the Ad5 genome, and C and G for respectively G and C at positions 23001 and 23004 in the Ad5 genome, introducing silent mutations in the adenovirus pVII and DNA-binding protein coding sequences.
Ad5.CMVβgal DNA can be used as a control to monitor the efficiency of transfection and virus recovery. It will generate a nuclear-targeted β galactosidase-expressing adenovirus that might be useful in your future experiments. Sufficient DNA is provided for 5 transfections.
|Vector Map||Sequence||Product Information Sheet|
|Vector Map||Ad5CMVbGal_DNA_#ZC-01.txt (33.69 KB)||Product_Information_Ad5CMVbGal_DNA_#ZC-01.pdf (139.45 KB)|