Plasmid-isolated genome of an Ad5 replication-deficient vector containing a CMV-GFP expression cassette in place of the E1 region
- Used as positive control for virus recovery during transfections
- GFP expression is under the control of a CMV promoter and a bGH polyA signal
- Allows for at least 5 transfections
Ad5.CMV-GFP DNA is the plasmid-isolated genome of an Ad5-based adenovirus containing a CMV-GFP-bGHpA expression cassette in place of the E1 region, oriented from right to left relative to the adenovirus genome. The Dasher GFP coding sequence (ATUM # FPB-27-609) is under the control of a human cytomegalovirus (CMV) promoter and a bovine growth hormone polyadenylation signal. Both E1 (3.1 kb) and E3 (2.7 kb) regions are deleted.
Ad5.CMV-GFP DNA can be used to monitor the efficiency of transfection and virus recovery. It will generate a GFP-expressing adenovirus that might be useful in your future experiments. Sufficient DNA is provided for 5 transfections.
|Vector Map||Sequence||Product Information Sheet|
|Vector Map||Ad5.CMV-GFP-DNA-ZC02.txt (34.59 KB)||Product_Information_Ad5CMVGFP_DNA_ZC-02_Lot3604.pdf (139.86 KB)|