Shuttle plasmid for constructing adenovirus vectors with a 1.2 kb deletion in the E4 region

pAd1130-04 is a plasmid designed for constructing recombinant adenovirus vectors, in combination with the AdenoQuick2.0 plasmids pAd1127, pAd1128, pAd1129, and their derivatives. It is a derivative of pAd1130 with a 1.2 kb SmaI-BglII E4 deletion that encompasses ORF 1-4, and corresponds to bp 34119-35355 in the WT Ad5 genome.
pAd1130-04 includes the right ITR and a 1.9 kb-long fragment of the E4 region. The right ITR is flanked with PacI and SwaI sites.  The E4 region is terminated with two SfiI sites, which generate non-symmetrical sticky ends suitable for directional cloning. The plasmid contains a 5 kb stuffer made from scrambled phage λ DNA.  This stuffer increases the size of the ligation product of pAd1127, pAd1128, pAd1129, and pAd1130 so that it can be packaged efficiently into phage λ.