pAd292
Ad5 backbone for the construction of replication-deficient E1/E3-deleted dicistronic (a.k.a. bipartite) adenovirus vectors using the AdenoQuick1.0 system
- Transgene insertion in the E1 region using shuttle plasmid pE1.1
- Transgene insertion in the E3 region using shuttle plasmid pE3.1
- 2.7 kb E3 deletion
- Maximum 7.5 kb cargo capacity
- Pre-digested with SfiI
pAd292 (formerly known as AdenoQuick13.1 DNA) is a 36.7 kb plasmid that contains the sequences encompassing bp 3504-right end (9.8–100 mu) of the Ad5 genome. The two SfiI sites naturally present in WT Ad5 DNA were mutated by substituting A for G and C at positions 16291 and 16294 in the Ad5 genome, and C and G for respectively G and C at positions 23001 and 23004 in the Ad5 genome, introducing silent mutations in the adenovirus pVII and DNA-binding protein coding sequences. Two pairs of SfiI sites that allow for directional cloning replace the E1 and E3 regions. The size of the E3 region deletion is 2.7 kb. The right ITR is flanked by PacI and SwaI sites. pAd292 is used in combination with the shuttle vectors pE1.2 and pE3.1 to construct bipartite replication-deficient adenoviruses containing transgenes in place of the E1 and E3 regions. The maximum combined transgene capacity is 7.5 kb.
Product | Cat# | Size | Price | |
---|---|---|---|---|
pE1.2Shuttle plasmid for the construction of bipartite adenovirus vectors using the AdenoQuick1.0 system |
Cat# :
QP-02 |
20 ug |
$220.00 |
|
pE3.1Shuttle plasmid for the construction of bipartite adenovirus vectors using the AdenoQuick1.0 system |
Cat# :
QP-03 |
20 ug |
$220.00 |
Vector Map | Sequence | Product Information Sheet |
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Vector Map | pAd292.txt (47.82 KB) | Product_Information_pAd292.pdf (142.68 KB) |