Linear 32.5 kb Ad5 DNA for the construction of E1-deleted adenovirus vectors with the AdenoZAP2.0 cloning system (in vitro ligation or recombination)
- Insertion of heterologous sequences in place of the E1 region of the Ad5 genome
- WT E3
- Maximum cargo capacity: 5.2 kb
The RightZAP2.1 DNA is a 32.5 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is intact. The DNA was purified from a cosmid. It is used in combination with the shuttle vectors pZAP2.1 or pZAP2.2 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity is 5.2 kb.
|Vector Map||RightZAP21.txt (32.75 KB)|