Linear 29.8 kb Ad5 DNA for the construction of E1/E3-deleted adenovirus vectors with the AdenoZAP2.0 cloning system (in vitro ligation or recombination)
- Insertion of heterologous sequences in place of the E1 region of the Ad5 genome
- 2.7 kb E3 deletion
- Maximum cargo capacity: 7.9 kb
RightZAP2.2 DNA is a 29.8 kb linear DNA fragment that encompasses bp 3504-right end of the Ad5 genome. The E3 region is deleted (2.7 kb). The DNA was purified from a cosmid. It is used in combination with the shuttle vectors pZAP2.1 or pZAP2.2 to construct replication-deficient adenoviruses containing transgenes in place of the E1 region of the Ad5 genome. The maximum transgene capacity of the resulting virus is 7.9 kb.
|Vector Map||Sequence||Product Information Sheet|
|Vector Map||RightZAP22.txt (30.06 KB)||Product Information RightZAP22.pdf (209.83 KB)|